Reporter
Part:BBa_K511100
Designed by: Grant Robinson Group: iGEM11_MIT (2011-09-25)
EBFP2 MammoBlock
An engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces blue. Although this protein has enhanced fluorescence compared to previous blue fluorescent proteins, it still fluoresces significantly more weakly than other common reporters such as EYFP or mKate.
Figures 1 and 2 show typical levels of EBFP2-mediated fluorescence following transfection of HEK-293 cells with EBFP2 driven by the Hef1a low-level constitutive promoter.
For more information, consult the MIT iGEM 2011 wiki here.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | EBFP2 MammoBlock |